Title: Analysis of the Local Lymph Node Assay (LLNA) variability for assessing the prediction of skin sensitisation potential and potency of chemicals with non-animal approaches
Authors: DUMONT CORALIEVIEGAS BARROSO JOAO FILIPEMATYS IZABELAWORTH AndrewCASATI Silvia
Citation: TOXICOLOGY IN VITRO vol. 34 p. 220-228
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Publication Year: 2016
JRC N°: JRC100088
ISSN: 0887-2333
URI: http://www.sciencedirect.com/science/article/pii/S0887233316300753
http://publications.jrc.ec.europa.eu/repository/handle/JRC100088
DOI: 10.1016/j.tiv.2016.04.008
Type: Articles in periodicals and books
Abstract: The knowledge of the biological mechanisms leading to the induction of skin sensitisation has favoured in recent years the development of alternative non-animal methods. During the formal validation process, results from the Local Lymph Node Assay (LLNA) are generally used as reference data to assess the predictive capacity of the non-animal tests. This study reports an analysis of the variability of the LLNA for a set of chemicals for which multiple studies are available and considering three classification schemes: POS/NEG, GHS/CLP and ECETOC. As the type of vehicle used in a LLNA study is known to influence to some extent the results, two analyses were performed: considering the solvent used to test the chemicals and without considering the solvent. The results show that the number of discordant classifications increases when chemical is tested in more than one solvent. Moreover, it can be concluded that study results leading to classification in the strongest classes (1A and EXT) seem to be more reliable than those in the weakest classes. This study highlights the importance of considering the variability of the reference when evaluating non-animal tests.
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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