Title: The Virtual Cell Based Assay: current status and future perspectives
Authors: GRAEPEL RABEALAMON LARAASTURIOL BOFILL DAVIDBERGGREN ELISABETJOOSSENS ELISABETHPAINI ALICIAPRIETO PERAITA MARIA DEL PILARWHELAN MAURICEWORTH ANDREW
Citation: TOXICOLOGY IN VITRO vol. 45 no. part 2 p. 258-267
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Publication Year: 2017
JRC N°: JRC100237
ISSN: 0887-2333
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC100237
DOI: 10.1016/j.tiv.2017.01.009
Type: Articles in periodicals and books
Abstract: In order to replace the use of animals in toxicity testing, there is a need to predict in vivo toxic doses from concentrations that cause toxicological effects in relevant in vitro systems. The Virtual Cell Based Assay (VCBA) estimates time-dependent concentration of a test chemical in the cell and cell culture for a given in vitro system. The concentrations in the different compartments of the cell and test system are derived from ordinary differential equations, physicochemical parameters of the test chemical and properties of the cell line. The VCBA has been developed for a range of cell lines including BALB/c 3T3 cells, HepG2, HepaRG, lung A459 cells, and cardiomyocytes. The model can be used to design and refine in vitro experiments and extrapolate in vitro effective concentrations to in vivo doses that can be applied in risk assessment. In this paper, we first discuss potential applications of the VCBA: i) design of in vitro High Throughput Screening (HTS) experiments; ii) hazard identification (based on acute systemic toxicity); and iii) risk assessment. Further extension of the VCBA is discussed in the second part, exploring potential application to i) manufactured nanomaterials, ii) additional cell lines and endpoints, and considering iii) other opportunities.
JRC Directorate:Health, Consumers and Reference Materials

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