Title: A greener, quick and comprehensive extraction approach for LC-MS of multiple mycotoxins
Authors: BREIDBACH Andreas
Citation: TOXINS vol. 9 no. 91 p. 1-14
Publisher: MDPI AG
Publication Year: 2017
JRC N°: JRC106009
ISSN: 2072-6651
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC106009
DOI: 10.3390/toxins9030091
Type: Articles in periodicals and books
Abstract: In food / feed control mycotoxin analysis is often still performed "one analyte at a time". Here a method is presented which aims at making mycotoxin analysis environmentally friendlier through replacing acetonitrile by ethyl acetate and reducing chemical waste production by analyzing 4 mycotoxins together, forgoing sample extract clean-up, and minimizing solvent consumption. For this, 2 g of test material were suspended in 8 mL water and 16 mL ethyl acetate were added. Extraction was accelerated through sonication for 30 min and subsequent addition of 8 g sodium sulfate. After centrifugation 500 µL supernatant were spiked with isotopologues, dried down, reconstituted in mobile phase, and measured with LC-MS. The method was validated in-house and through a collaborative study and the performance was fit-for-purpose. Repeatability RSDs between 16 % at low and 4 % at higher contaminations were obtained. The reproducibility RSDs were mostly between 12 % and 32 %. The trueness of results for T-2 toxin and Zearalenone were not different from 100 %, for Deoxynivalenol and HT-2 toxin they were larger than 89%. The extraction was also adapted to a quick screening of aflatoxin B1 in maize by flow injection-MS. Semi-quantitative results were obtained through standard addition and scan-based ion ratio calculations. The method proved to be a viable greener and quicker alternative to existing methods.
JRC Directorate:Health, Consumers and Reference Materials

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