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|Title:||Animal Fat Discrimination: Investigation of the Potential of FT-IR, GC, Immuno-assay and PCR Techniques|
|Authors:||VON HOLST CHRISTOPH; BELLORINI STEFANO; STRATMAN Stefan; TIRENDI SALVATORE; BAETEN Vincent; BERBEN Gilbert; FUMIÈRE Olivier|
|Citation:||ANALYTICAL AND BIOANALYTICAL CHEMISTRY (FORMERLY FRESENIUS) no. 382 p. 1073-1083|
|JRC Publication N°:||JRC30322|
|Type:||Articles in Journals|
|Abstract:||The objective of the reported study the identificaiton of ruminant fat and its differentiation from non-ruminant fats. Four different techniques were compared in terms of their suitability for enforcing existing and up-coming legislation on animal by-products. The performance for the identificaiton of the animal fats origin (1) Fourier Transform Infrared Spectrscopy (FT-IR) applied on the fat samples, (2) Gas chromatography coupled with Mass Spectrometry (GC-MS) to determine the fatty acid profile, (3) immuno-assays focusing on the protein fraction included in fat and (4) polymerase chain reaction (PCR) for the detection of bovine specific DNA were evaluated. The immunoassay and PCR techniques turned out to be fit for the purpose whereas the FT-NIR and GC-MS only worked on pure samples but not on mixtures.|
|JRC Institute:||Institute for Reference Materials and Measurements|
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