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|Title:||Effect of Roasting on the Radical Scavenging Activity of Cocoa Beans|
|Authors:||SUMMA Carmelina; CORDEIRO RAPOSO FERNANDO; MCCOURT JOSEPHINE; ANKLAM ELKE; LO SCALZO Roberto; WAGNER Karl-Heinz; ELMADFA Ibrahim|
|Citation:||EUROPEAN FOOD RESEARCH AND TECHNOLOGY vol. 222 no. 3-4 p. 368-375|
|Type:||Articles in periodicals and books|
|Abstract:||The free radical scavenging activity of cocoa samples subjected to different roasting treatments has been determined. The samples (raw, pre-roasted and roasted) were separated into four molecular weight fractions per sample (>30 kDa, 30-10 kDa, 10-5 kDa, <5 kDa). The free radical scavenging activity was determined with DPPHx, and ABTSx+ free radical scavenging assays for all samples. Both tests were compared in terms of sensitivity and measurement precision, at different reaction times. Comparing the results from each test, the free radical scavenging activity trends were similar for each fraction but with notable differences in the sensitivity of the assays. Analysis of the concentration of reducing substances such as water soluble phenolics, melanoidins, carbohydrates etc … in these fractions by the photometric Folin-Ciocalteu assay, showed a similar pattern to the free radical scavenging activity trend. Moreover, this comparison showed that there were significantly (p<0.05) more reducing substances and free radical scavenging activity in the 10 -5 kDa roasted cocoa bean fraction.|
|JRC Institute:||Institute for Reference Materials and Measurements|
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