Title: Effect of Roasting on the Radical Scavenging Activity of Cocoa Beans
Citation: EUROPEAN FOOD RESEARCH AND TECHNOLOGY vol. 222 no. 3-4 p. 368-375
Publisher: SPRINGER
Publication Year: 2006
JRC N°: JRC31030
ISSN: 1438-2377
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC31030
Type: Articles in periodicals and books
Abstract: The free radical scavenging activity of cocoa samples subjected to different roasting treatments has been determined. The samples (raw, pre-roasted and roasted) were separated into four molecular weight fractions per sample (>30 kDa, 30-10 kDa, 10-5 kDa, <5 kDa). The free radical scavenging activity was determined with DPPHx, and ABTSx+ free radical scavenging assays for all samples. Both tests were compared in terms of sensitivity and measurement precision, at different reaction times. Comparing the results from each test, the free radical scavenging activity trends were similar for each fraction but with notable differences in the sensitivity of the assays. Analysis of the concentration of reducing substances such as water soluble phenolics, melanoidins, carbohydrates etc … in these fractions by the photometric Folin-Ciocalteu assay, showed a similar pattern to the free radical scavenging activity trend. Moreover, this comparison showed that there were significantly (p<0.05) more reducing substances and free radical scavenging activity in the 10 -5 kDa roasted cocoa bean fraction.
JRC Directorate:Health, Consumers and Reference Materials

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