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|Title:||Development of an innovative immunoassay for CP4EPSPS and Cry1AB GM proteins detection and quantification|
|Authors:||ERMOLLI MONICA; PROSPERO ADELINA; BALLA BRANKO; QUERCI MADDALENA; MAZZEO Alessandra; VAN DEN EEDE GUY|
|Citation:||FOOD ADDITIVES AND CONTAMINANTS vol. 23 no. 9 p. 876-882|
|Publisher:||TAYLOR & FRANCIS LTD|
|JRC Publication N°:||JRC32181|
|Type:||Articles in Journals|
|Abstract:||An innovative immunoassay, called ELISA Reverse, based on a new conformation of the solid phase was developed; the solid support was expressly designed for being immersed directly in liquid samples to detect the presence of protein targets. Its application is proposed in those cases where a large number of samples have to be screened simultaneously or when the simultaneous detection of different proteins is required. As a first application a quantitative immunoassay for Cry1AB protein in GM maize was optimised. The method was tested using GMO concentrations from 0.1 to 2%. The LOD and LOQ of the method were respectively determined equal to 0.0056 and 0.0168 (expressed in % of GMO content). A protocol to assess the presence of two GM proteins simultaneously has also been established for the case of the Cry1AB and the CP4EPSPS respectively present in GM maize and soy.|
|JRC Institute:||Institute for Health and Consumer Protection|
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