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|Title:||Apolipoprotein AI Could be a Significant Determinant of Epithelial Integrity in Rainbow Trout Gill Cell Cultures:a Study in Functional Proteomics|
|Authors:||SMITH Richard W.; WOOD Chris M.; CASH Phil; DIAO Linda; PART PETER|
|Citation:||BIOCHIMICA ET BIOPHYSICA ACTA vol. 1749 p. 81-93|
|Publisher:||ELSEVIER SCIENCE BV|
|Type:||Articles in periodicals and books|
|Abstract:||Abstract The fresh water fish gill forms a barrier against an external hypotonic environment. By culturing rainbow trout gill cells on permeable supports, as intact epithelia, this study investigates barrier property mechanisms. Under symmetrical conditions the apical and basolateral epithelial surfaces contact cell culture media. Replacing apical media with water, to generate a symmetrical conditions(i.e. the situation encountered by the fresh watergill), rapidly increases transepithelial resistance(TER). Proteomic analysis revealed that this is associated with enhanced expression of pre-apolipoprotein AI (pre- apoAI). To test the physiological relevance, gill cells were treated with a dose of 50 Ag ml 1human apolipoprotein(apoAI). This was found to elevate TER in those epithelia which displayed a lower TER prior to apoAI treatment. These results demonstrate the action of apoAI and provide evidence that the rainbow trout gill maybe a site of apoAI synthesis. TER does not differentiate between the trans-cellular (via the cell membrane) and para-cellular (via inter cellular tight junctions) pathways. However, despite the apoAI - induced changes in TER, para-cellular permeability (measured by polyethylene glycol efflux)remained unaltered suggesting apoAI specifically reduce strans-cellular permeability. This investigation combines proteomics with functional measurements to show how a proteome change maybe associated with fresh water gill function.|
|JRC Institute:||Institute for Environment and Sustainability|
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