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|Title:||Determination of Aflatoxin B1 in Tiger Nut-based Soft Drinks|
|Authors:||ARRANZ HERNANDEZ ISABEL; STROKA JOERG; NEUGEBAUER L.|
|Citation:||FOOD ADDITIVES AND CONTAMINANTS vol. 23 no. 3 p. 305-308|
|Publisher:||TAYLOR & FRANCIS LTD|
|JRC Publication N°:||JRC33204|
|Type:||Articles in Journals|
|Abstract:||An analytical method for the determination of aflatoxin B1 in a tiger nut-based soft drinks named ‘horchata’ is described. The method is based on an immunoaffinity clean-up, followed by HPLC separation and fluorescence detection after electrochemical post-column derivatization. The detection limit (S/N?=?3) and the quantification limit (S/N?=?10) were 0.02 and 0.06?µg?kg-1, respectively. The mean recovery at a level of 2?µg?l-1 was 88% (n?=?6) and the coefficient of variation was 9%. The method was applied to conduct a small market survey for a beverage named ‘horchata’ that is frequently consumed by parts of the population in Southern Europe. Twenty-two samples from Spanish and Belgian supermarkets were analysed. As a result, only one sample was found to contain aflatoxin B1 at the limit of quantitation of the method.|
|JRC Institute:||Institute for Reference Materials and Measurements|
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