Title: Determination of Aflatoxin B1 in Tiger Nut-based Soft Drinks
Authors: ARRANZ HERNANDEZ ISABELSTROKA JOERGNEUGEBAUER L.
Citation: FOOD ADDITIVES AND CONTAMINANTS vol. 23 no. 3 p. 305-308
Publisher: TAYLOR & FRANCIS LTD
Publication Year: 2006
JRC N°: JRC33204
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC33204
Type: Articles in Journals
Abstract: An analytical method for the determination of aflatoxin B1 in a tiger nut-based soft drinks named ‘horchata’ is described. The method is based on an immunoaffinity clean-up, followed by HPLC separation and fluorescence detection after electrochemical post-column derivatization. The detection limit (S/N?=?3) and the quantification limit (S/N?=?10) were 0.02 and 0.06?µg?kg-1, respectively. The mean recovery at a level of 2?µg?l-1 was 88% (n?=?6) and the coefficient of variation was 9%. The method was applied to conduct a small market survey for a beverage named ‘horchata’ that is frequently consumed by parts of the population in Southern Europe. Twenty-two samples from Spanish and Belgian supermarkets were analysed. As a result, only one sample was found to contain aflatoxin B1 at the limit of quantitation of the method.
JRC Institute:Institute for Reference Materials and Measurements

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