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|Title:||Effective PCR Detection of Animal Species in highly processed Animal By-Products and Compund Feeds|
|Authors:||BAETEN Vincent; BERBEN Gilbert; FUMIERE O.; VON HOLST CHRISTOPH|
|Citation:||ANALYTICAL AND BIOANALYTICAL CHEMISTRY (FORMERLY FRESENIUS) vol. 385 p. 1045-1054|
|JRC Publication N°:||JRC33449|
|Type:||Articles in Journals|
|Abstract:||In this papaer we present a method based on Polymerase Chain Reaction (PCR) to detect meat and bone meal (MBM) in compound feedingstuffs. By choosing adequate DNA targets from an appropriate localisation in the genome the developed Real Time PCR method proved to be robust against severe heat treatment of the MBM, showing high sensitivity for the detectin of MBM. The mothod developed allows a specific detection of pig and cattle processed materials treated at 134°C in various feed matrices with a limit of detection of about 0.1%. This technique has also been successfully applied on well-characterised MBM samples treated as far as 141 ºC as well as on various blind feed samples with very low MBM content. Finally the methods also passed successfully several official European ring trials.|
|JRC Institute:||Institute for Reference Materials and Measurements|
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