Title: Immobilization of Antibodies on Biosensing Devices by Nanoarrayed Self-Assembled Monolayers
Authors: VALSESIA ANDREACOLPO PascalMEZIANI TarikVIEIRA LISBOA PATRICIALEJEUNE MichaelROSSI FRANCOIS
Citation: LANGMUIR vol. 22 p. 1763-1767
Publisher: AMER CHEMICAL SOC
Publication Year: 2006
JRC N°: JRC33495
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC33495
Type: Articles in Journals
Abstract: This work presents an original and straightforward technique for antibody immobilization onto a surface, keeping the antibody in a biologically reactive configuration. Self-assembly of molecular monolayers and plasma-based colloidal lithography were combined to create chemical nanopatterns on the surface of a biosensing device. This technique was employed to create an array of 100 nm wide motifs having a hexagonal 2-D crystalline structure, characterized by COOH-terminated nanospots in a CH3-terminated matrix. The quality control of the chemical nanopattern was carried out by combining atomic force microscopy, ellipsometry, and contact angle measurements. Enzyme-linked immunosorbent assay experiments were set up showing that the COOH/CH3 nanopatterned surface constrains the immobilization of the antibodies in a biologically reactive configuration, thus significantly improving the device performances as compared to those of more conventional nanopatterned COOH-terminated or CH3-terminated surfaces.
JRC Institute:Institute for Health and Consumer Protection

Files in This Item:
There are no files associated with this item.


Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.