Title: First Application of a Micro-sphere Based Immunoassay to GMO Detection: Quantification of Cry1Ab Protein in GM Maize
Authors: FANTOZZI AnnaERMOLLI MONICAMARINI MASSIMILIANOSCOTTI DomenicoBALLA BRANKOQUERCI MADDALENALANGRELL STEPHENVAN DEN EEDE GUY
Citation: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY vol. 55 p. 1071-1076
Publisher: AMER CHEMICAL SOC
Publication Year: 2007
JRC N°: JRC33527
URI: http://pubs.acs.org/doi/abs/10.1021/jf061506p
http://publications.jrc.ec.europa.eu/repository/handle/JRC33527
Type: Articles in Journals
Abstract: An innovative covalent micro-sphere immunoassay, based on the usage of fluorescent beads coupled to a specific antibody, was developed for the quantification of the endotoxin Cry1Ab present in MON810 and Bt11 GM maize lines. In particular a specific protocol was developed to asses the presence of Cry1Ab in a very broad range of GM maize concentrations, from 0.1 to 100% (weight GMO/weight). Test linearity was achieved in the range of values from 0.1 to 3% while fluorescence saturation level occurred at 25%. The limit of detection (LOD) and quantification (LOQ) were equal to 0.018% and 0.054% respectively. The present study describes the first application of quantitative high trough put immunoassays in GMO analysis.
JRC Institute:Institute for Health and Consumer Protection

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