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|Title:||First Application of a Micro-sphere Based Immunoassay to GMO Detection: Quantification of Cry1Ab Protein in GM Maize|
|Authors:||FANTOZZI Anna; ERMOLLI MONICA; MARINI MASSIMILIANO; SCOTTI Domenico; BALLA BRANKO; QUERCI MADDALENA; LANGRELL STEPHEN; VAN DEN EEDE GUY|
|Citation:||JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY vol. 55 p. 1071-1076|
|Publisher:||AMER CHEMICAL SOC|
|Type:||Articles in periodicals and books|
|Abstract:||An innovative covalent micro-sphere immunoassay, based on the usage of fluorescent beads coupled to a specific antibody, was developed for the quantification of the endotoxin Cry1Ab present in MON810 and Bt11 GM maize lines. In particular a specific protocol was developed to asses the presence of Cry1Ab in a very broad range of GM maize concentrations, from 0.1 to 100% (weight GMO/weight). Test linearity was achieved in the range of values from 0.1 to 3% while fluorescence saturation level occurred at 25%. The limit of detection (LOD) and quantification (LOQ) were equal to 0.018% and 0.054% respectively. The present study describes the first application of quantitative high trough put immunoassays in GMO analysis.|
|JRC Institute:||Institute for Health and Consumer Protection|
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