Title: Towards Metrological Traceability for DNA Fragment Ratios in GM Quantification 2. Systematic Study of Parameters Influencing the Quantitative Determination of MON 810 Corn by Real-time PCR
Authors: CHARELS DIANABROEDERS SYLVIACORBISIER PHILIPPETRAPMANN STEFANIESCHIMMEL HEINZLINSINGER THOMASEMONS HENDRIK
Citation: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY vol. 55 p. 3258-3267
Publisher: AMER CHEMICAL SOC
Publication Year: 2007
JRC Publication N°: JRC34999
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC34999
Type: Articles in Journals
Abstract: The current paper is part of a set of three papers investigating metrological traceability of the quantification of DNA fragments. This papaer evaluates the possible impact of several factors like particle size of the unknown powder samples, the DNA extraction method, the nature of the calibrant and the real-time Polymerase Chain Reaction detection method. The results obtained in this interlaboratory comparison revealed a significant influence of the DNA extraction method on the measurement results when the construct-specific real-time PCR detection method was applied. Statistical analyses confirmed the importance of validating DNA extraction methods in conjunction with real)time PCR methods.
JRC Institute:Institute for Reference Materials and Measurements

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