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|Title:||Towards Metrological Traceability for DNA Fragment Ratios in GM Quantification 2. Systematic Study of Parameters Influencing the Quantitative Determination of MON 810 Corn by Real-time PCR|
|Authors:||CHARELS DIANA; BROEDERS SYLVIA; CORBISIER PHILIPPE; TRAPMANN STEFANIE; SCHIMMEL HEINZ; LINSINGER THOMAS; EMONS HENDRIK|
|Citation:||JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY vol. 55 p. 3258-3267|
|Publisher:||AMER CHEMICAL SOC|
|Type:||Articles in Journals|
|Abstract:||The current paper is part of a set of three papers investigating metrological traceability of the quantification of DNA fragments. This papaer evaluates the possible impact of several factors like particle size of the unknown powder samples, the DNA extraction method, the nature of the calibrant and the real-time Polymerase Chain Reaction detection method. The results obtained in this interlaboratory comparison revealed a significant influence of the DNA extraction method on the measurement results when the construct-specific real-time PCR detection method was applied. Statistical analyses confirmed the importance of validating DNA extraction methods in conjunction with real)time PCR methods.|
|JRC Institute:||Institute for Reference Materials and Measurements|
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