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|Title:||A Proteomics-based Approach to Detect and Identify Major Allergens in Processed Peanut by Capillary LC-Q-TOF (MS/MS)|
|Authors:||CHASSAIGNE HUBERT; NORGAARD JORGEN; VAN HENGEL ADRIANUS|
|Citation:||JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY vol. 55 p. 4461-4473|
|Publisher:||AMER CHEMICAL SOC|
|JRC Publication N°:||JRC35811|
|Type:||Articles in Journals|
|Abstract:||An MS-based method, combining reverse-phase capillary liquid chromatography (capillary LC) with quadrupole-time-of-flight tandem mass spectrometry (nano-ESI Q-TOP MS/MS) was developed with the aim to identify a set of peptides that can function as markers for peanut allergens. Emphasis was given to the identification of the three major peanut allergens Ara h1, Ara h 2 and Ara h 3, since these proteins are considered to represent more than 30% of the total protein content of peanut and are directly relevant for the allergenic potential of this food.|
|JRC Institute:||Institute for Reference Materials and Measurements|
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