Title: A Proteomics-based Approach to Detect and Identify Major Allergens in Processed Peanut by Capillary LC-Q-TOF (MS/MS)
Authors: CHASSAIGNE HUBERTNORGAARD JORGENVAN HENGEL ADRIANUS
Citation: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY vol. 55 p. 4461-4473
Publisher: AMER CHEMICAL SOC
Publication Year: 2007
JRC Publication N°: JRC35811
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC35811
Type: Articles in Journals
Abstract: An MS-based method, combining reverse-phase capillary liquid chromatography (capillary LC) with quadrupole-time-of-flight tandem mass spectrometry (nano-ESI Q-TOP MS/MS) was developed with the aim to identify a set of peptides that can function as markers for peanut allergens. Emphasis was given to the identification of the three major peanut allergens Ara h1, Ara h 2 and Ara h 3, since these proteins are considered to represent more than 30% of the total protein content of peanut and are directly relevant for the allergenic potential of this food.
JRC Institute:Institute for Reference Materials and Measurements

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