Title: D-alanyl Ester Depletion of Teichoic Acids in Lactobacillus Plantarum Results in a Major Modification of Lipoteichoic Acid Composition and Cell Wall Perforations at the Septum Mediated by the Acm2 Autolysin
Authors: PALUMBO EmmanuelleDEGHORAIN MarieCOCCONCELLI Pier SandroKLEEREBEZEM MichielGEYER ArminHARTUNG THOMASMORATH SIEGFRIEDHOLS Pascal
Citation: JOURNAL OF BACTERIOLOGY vol. 188 no. 10 p. 3709-3715
Publisher: AMER SOC MICROBIOLOGY
Publication Year: 2006
JRC N°: JRC35942
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC35942
Type: Articles in Journals
Abstract: The insertional inactivation of the dlt operon from Lactobacillus plantarum NCIMB8826 had a strong impact on lipoteichoic acid (LTA) composition, resulting in a major reduction in D-alanyl ester content. Unexpectedly, mutant LTA showed high levels of glucosylation and were threefold longer than wild-type LTA. The dlt mutation resulted in a reduced growth rate and increased cell lysis during the exponential and stationary growth phases. Microscopy analysis revealed increased cell length, damaged dividing cells, and perforations of the envelope in the septal region. The observed defects in the separation process, cell envelope perforation, and autolysis of the dlt mutant could be partially attributed to the L. plantarum Acm2 peptidoglycan hydrolase.
JRC Institute:Institute for Health and Consumer Protection

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