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|Title:||D-alanyl Ester Depletion of Teichoic Acids in Lactobacillus Plantarum Results in a Major Modification of Lipoteichoic Acid Composition and Cell Wall Perforations at the Septum Mediated by the Acm2 Autolysin|
|Authors:||PALUMBO Emmanuelle; DEGHORAIN Marie; COCCONCELLI Pier Sandro; KLEEREBEZEM Michiel; GEYER Armin; HARTUNG THOMAS; MORATH SIEGFRIED; HOLS Pascal|
|Citation:||JOURNAL OF BACTERIOLOGY vol. 188 no. 10 p. 3709-3715|
|Publisher:||AMER SOC MICROBIOLOGY|
|Type:||Articles in periodicals and books|
|Abstract:||The insertional inactivation of the dlt operon from Lactobacillus plantarum NCIMB8826 had a strong impact on lipoteichoic acid (LTA) composition, resulting in a major reduction in D-alanyl ester content. Unexpectedly, mutant LTA showed high levels of glucosylation and were threefold longer than wild-type LTA. The dlt mutation resulted in a reduced growth rate and increased cell lysis during the exponential and stationary growth phases. Microscopy analysis revealed increased cell length, damaged dividing cells, and perforations of the envelope in the septal region. The observed defects in the separation process, cell envelope perforation, and autolysis of the dlt mutant could be partially attributed to the L. plantarum Acm2 peptidoglycan hydrolase.|
|JRC Directorate:||Institute for Health and Consumer Protection Historical Collection|
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