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|Title:||Controlled Micropatterning of Biomolecules for Cell Culturing|
|Authors:||RUIZ ANA MARIA; CERIOTTI LAURA; BUZANSKA LEONORA; HASIWA MARINA; BRETAGNOL FREDERIC; CECCONE GIACOMO; GILLILAND DOUGLAS; RAUSCHER HUBERT; COECKE SANDRA; COLPO PASCAL; ROSSI FRANCOIS|
|Citation:||MICROELECTRONIC ENGINEERING vol. 84 no. 5-8 p. 1733-1736|
|Publisher:||ELSEVIER SCIENCE BV|
|Type:||Articles in Journals|
|Abstract:||Various biomolecules have been patterned on anti-fouling substrates (Poly-ethylene glycol, PEG, plasma polymerized) by microcontact printing. Photolithography and reactive ion etching have been used to fabricate the microstructured silicon moulds needed for the production of stamps. Replicas of the microsized features were created in polydimethyl siloxane. The elastomeric stamps were used for micropatterning different biomolecules on PEG. Characterisation by Fluorescence Microscopy, ToF-SIMS and Ellipsometry showed well-defined stable patterns. Incubation of the samples with solutions of fibroblasts and neural stem cells highlighted how the cells clearly adhered and proliferate in the regions covered with the biomolecules.|
|JRC Institute:||Institute for Health and Consumer Protection|
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