Title: Controlled Micropatterning of Biomolecules for Cell Culturing
Authors: RUIZ ANA MARIACERIOTTI LAURABUZANSKA LEONORAHASIWA MARINABRETAGNOL FREDERICCECCONE GIACOMOGILLILAND DOUGLASRAUSCHER HUBERTCOECKE SANDRACOLPO PASCALROSSI FRANCOIS
Citation: MICROELECTRONIC ENGINEERING vol. 84 no. 5-8 p. 1733-1736
Publisher: ELSEVIER SCIENCE BV
Publication Year: 2007
JRC Publication N°: JRC36262
ISSN: 0167-9317
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC36262
DOI: 10.1016/j.mee.2007.01.200
Type: Articles in Journals
Abstract: Various biomolecules have been patterned on anti-fouling substrates (Poly-ethylene glycol, PEG, plasma polymerized) by microcontact printing. Photolithography and reactive ion etching have been used to fabricate the microstructured silicon moulds needed for the production of stamps. Replicas of the microsized features were created in polydimethyl siloxane. The elastomeric stamps were used for micropatterning different biomolecules on PEG. Characterisation by Fluorescence Microscopy, ToF-SIMS and Ellipsometry showed well-defined stable patterns. Incubation of the samples with solutions of fibroblasts and neural stem cells highlighted how the cells clearly adhered and proliferate in the regions covered with the biomolecules.
JRC Institute:Institute for Health and Consumer Protection

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