Title: Metabolomics Applied to Exhaled Breath Condensate in Childhood Asthma
Authors: CARRARO SilviaREZZI SergeRENIERO FABIANOHEBERGER KarolyGIORDANO GiuseppeZANCONATO StefaniaGUILLOU CLAUDEBARALDI Eugenio
Citation: AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE vol. 175 p. 986-990
Publisher: AMER THORACIC SOC
Publication Year: 2007
JRC Publication N°: JRC36846
ISSN: 1073-449X
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC36846
DOI: 10.1164/rccm.200606-769OC
Type: Articles in Journals
Abstract: The discrimination of healthy and asthmatic cases can be solved using linear- and partial least squares discrimninant analysis of clinical and metabonomics (NMR) data. 25 asthmatic children and 11 healthy children were enrolled in the study. For each child the EBC NMR spectrum and three clinical variables were determined. Although the number of cases did not allow of applying external validation or cross validation the present data makes the following conclusions possible: Linear Discriminant Analysis (LDA) using best subset selection has found that the best distinction between asthmatic and healthy children is obtained from a four variable model including FEV1 and chemical shifts at 2.02, 1.82, 1.74 in the NMR spectrum. Taken together the 4 variables discriminate asthmatic from healthy children with a success rate of up to 97%. This result points out how the best characterization of asthmatic subjects comes from the combined knowledge of lung function and ongoing inflammatory pattern. Being in the 1.8-2.3 range within the NMR spectrum, the peaks included in the best model are likely related to the presence of acetylated products in asthmatic airways. Our finding of acetylated products in asthmatic children open the way toward further studies aiming at the identification of these products, among which HMGB1 could have a role
JRC Institute:Institute for Health and Consumer Protection

Files in This Item:
There are no files associated with this item.


Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.