Title: Surface and Bioproperties of Nanocrystalline Diamond/Amorphous Carbon Nanocomposite Films
Authors: KULISCH WILHELMPOPOV CYRILBLIZNAKOV S.CECCONE GIACOMOGILLILAND DOUGLASRAUSCHER HUBERTSIRGHI LUCELROSSI FRANCOIS
Citation: THIN SOLID FILMS vol. 515 no. 23 p. 8407-8411
Publisher: ELSEVIER SCIENCE SA
Publication Year: 2007
JRC N°: JRC36878
ISSN: 0040-6090
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC36878
DOI: 10.1016/j.tsf.2007.05.054
Type: Articles in Journals
Abstract: Nanocrystalline diamond/amorphous carbon nanocomposite films have been deposited by microwave plasma chemical vapour deposition from CH4/N2 mixtures. Their bulk properties have been investigated, among others, by XRD, Raman spectroscopy and FTIR. They consist of diamond nanocrystals of 3-5 nm diameter, embedded in an amorphous carbon matrix with a crystallite/matrix ratio of about unity. In order to assess the suitability of such NCD/a-C films for application in biotechnology and biosensorics, e.g. as a template for the immobilization of biomolecules, the properties of four differently prepared surfaces, namely two hydrophobic (as-grown and plasma hydrogen terminated) and two hydrophilic (plasma and chemically oxygen terminated) were thoroughly investigated by means of X-ray photoelectron spectroscopy, contact angle measurements, zeta-potential measurements and atomic force microscopy. By performing measurements as a function of time, the stability of the different surfaces was established. Cytotoxicity and simulated body fluid tests revealed that as-grown NCD/a-C surfaces are not cytotoxic and bioinert. Finally, the non-specific absorption of biomolecules such as proteins and RNA (biofouling) on the various surfaces was investigated by AFM, scanning force spectroscopy, TOF-SIMS and ellipsometry. It could be shown that the non-specific absorbtion on hydrophobic surfaces is very low as compared to control materials such as mica or glas.
JRC Institute:Institute for Health and Consumer Protection

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