Title: Heterodyne Interference Microscopy for Non-Invasive Cell Morphometry
Authors: LAKESTANI FEREYDOUNREMBGES DIANASACCO MARIA-GRAZIA
Other Contributors: WHELAN MAURICE
Citation: SPIE Proceedings vol. 6631 p. Paper 14
Publisher: SPIE
Publication Year: 2007
JRC N°: JRC37507
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC37507
Type: Contributions to Conferences
Abstract: This paper describes two setups suitable for interference microscopy for high resolution morphological measurements on living cells in culture medium. The first system incorporated a PZT actuator in the reference path of a Mach Zehnder configuration to facilitate digital phase-stepping interferometry. The second system employed two hase-locked acoustooptic modulators to generate a temporal optical carrier to allow a eterodyne approach to phase demodulation. This setup incorporated a digital CMOS camera with full random pixel access which allowed the heterodyne approach to be implemented as a full-field method without any need for electromechanical scanning. The heterodyne approach offers benefits over the phase-stepping method in terms of measurement resolution and speed, typically offering the equivalent of nanometer resolution for cell height measurements with a bandwidth in the order of 200-300 Hz for 1000 pixels. Results for morphological measurements using both systems on red blood cells and keratinocytes are presented.
JRC Institute:Institute for Health and Consumer Protection

Files in This Item:
There are no files associated with this item.


Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.