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|Title:||Heterodyne Interference Microscopy for Non-Invasive Cell Morphometry|
|Authors:||LAKESTANI FEREYDOUN; REMBGES DIANA; SACCO MARIA-GRAZIA|
|Other Contributors:||WHELAN MAURICE|
|Citation:||SPIE Proceedings vol. 6631 p. Paper 14|
|Type:||Articles in periodicals and books|
|Abstract:||This paper describes two setups suitable for interference microscopy for high resolution morphological measurements on living cells in culture medium. The first system incorporated a PZT actuator in the reference path of a Mach Zehnder configuration to facilitate digital phase-stepping interferometry. The second system employed two hase-locked acoustooptic modulators to generate a temporal optical carrier to allow a eterodyne approach to phase demodulation. This setup incorporated a digital CMOS camera with full random pixel access which allowed the heterodyne approach to be implemented as a full-field method without any need for electromechanical scanning. The heterodyne approach offers benefits over the phase-stepping method in terms of measurement resolution and speed, typically offering the equivalent of nanometer resolution for cell height measurements with a bandwidth in the order of 200-300 Hz for 1000 pixels. Results for morphological measurements using both systems on red blood cells and keratinocytes are presented.|
|JRC Institute:||Institute for Health and Consumer Protection Historical Collection|
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