Title: Non-invasive Visualisation of the Development of Peritoneal Carcinomatosis and Tumour Regression After 213Bi-radioimmunotherapy Using Bioluminescence Imaging
Authors: BUCHHORN H.m.SEIDL C.BECK R.SAUR D.APOSTOLIDIS CHRISTOSMORGENSTERN ALFREDSCHWAIGER M.SENEKOWITSCH-SCHMIDTKE R.
Citation: EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING vol. 34 p. 841-849
Publisher: Springer Verlag
Publication Year: 2007
JRC N°: JRC38135
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC38135
Type: Articles in Journals
Abstract: Purpose Non-invasive imaging of tumour development remains a challenge, especially for tumours in the intraperitoneal cavity. Therefore, the aim of this study was the visualisation of both the development of peritoneal carcinomatosis and tumour regression after radioimmunotherapy with tumour-specific 213Bi-Immunoconjugates, via in vivo bioluminescence imaging of firefly luciferase-transfected cells. Methods Human diffuse-type gastric cancer cells expressing mutant d9-E-cadherin were stably transfected with firefly luciferase (HSC45-M2-luc). For bioluminescence imaging, nude mice were inoculated intraperitoneally with 1 × 107 HSC45-M2-luc cells. On days 4 and 8 after tumour cell inoculation, imaging was performed following D-luciferin injection using a cooled CCD camera with an image intensifier unit. For therapy, mice were injected with 2.7 MBq 213Bi-d9MAb targeting d9-E-cadherin on day 8 after tumour cell inoculation. Bioluminescence images were taken every 4 days to monitor tumour development.
JRC Institute:Institute for Transuranium Elements

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