Title: Molecular Interaction between LTAs and L. delbrueckii Phages Depends on D4 Alanyl and alfa-Glucose Substitution of Poly(glycerophosphate) Backbones
Authors: RÄISÄNEN LiisaDRAING C.PFITZENMAIER MarkusSCHUBERT KarinJAAKONSAARI TiinaVON AULOCK SONJAHARTUNG THOMASALATOSSAVA Tapani
Citation: JOURNAL OF BACTERIOLOGY vol. 189 no. 11 p. 4135-4140
Publisher: AMER SOC MICROBIOLOGY
Publication Year: 2007
JRC Publication N°: JRC38179
ISSN: 0021-9193
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC38179
DOI: 10.1128/JB.00078-07
Type: Articles in Journals
Abstract: Lipoteichoic acids (LTAs) have been shown to act as bacterial counterparts to the receptor binding proteins of LL-H, LL-H host range mutant LL-H-a21 and JCL1032. Here we have used HIC-purified LTAs from different phage resistant and sensitive strains of L. delbrueckii subsp. lactis. NMR analyses revealed variation in the degree of α-glucosyl and D-alanyl substitution of the 1,3-linked poly(glycerophosphate) LTAs between the phage sensitive and the phage resistant strains. Inactivation of phages was less effective if there was a high level of D-alanine residues in the LTA backbones. Prior incubation of the LTAs with α-glucose-specific lectin inhibited the LL-H phage inactivation. The overall level of decoration or the specific spatial combination of α-glucosyl substituted, D-alanyl substituted and nonsubstituted glycerol residues may also affect phage adsorption.
JRC Institute:Institute for Health and Consumer Protection

Files in This Item:
There are no files associated with this item.


Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.