Title: Primers and Polymerase Chain Reaction Conditions for DNA Barcoding Teleost Fish based on the Mitochondrial Cytochrome b and Nuclear Rhodopsin Genes.
Authors: BAUTISTA Jose M.SEVILLA Rafael G.DIEZ AmaliaNOREN MichaelMOUCHEL OlivierJEROME MarcVERREZ-BAGNIS VeroniqueVAN PELT HildeFAVRE-KREY LaurenceKREY GrigoriosCARREAU PhilippeKOURTI NAOUMAMARTINSOHN JANN
Citation: MOLECULAR ECOLOGY NOTES vol. 7 p. 730-734
Publisher: BLACKWELL PUBLISHING
Publication Year: 2007
JRC N°: JRC40292
ISSN: 1471-8278
URI: http://www.blackwell-synergy.com/doi/abs/10.1111/j.1471-8286.2007.01863.x
http://publications.jrc.ec.europa.eu/repository/handle/JRC40292
DOI: 10.1111/j.1471-8286.2007.01863.x
Type: Articles in Journals
Abstract: This report describes a set of 21 polymerase chain reaction primers and amplification conditions developed to barcode practically any teleost fish species according to their mitochondrial cytochrome b and nuclear rhodopsin gene sequences. The method was successfully tested in more than 200 marine fish species comprising the main Actinopteygii family groups. When used in phylogenetic analyses, its combination of two genes with different evolutionary rates serves to identify fish at the species level. We provide a flow diagram indicating our validated polymerase chain reaction amplification conditions for barcoding and species identification applications as well as population structure or haplotyping analyses, adaptable to high-throughput analyses.
JRC Institute:Institute for the Protection and Security of the Citizen

Files in This Item:
There are no files associated with this item.


Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.