Please use this identifier to cite or link to this item:
|Title:||Primers and Polymerase Chain Reaction Conditions for DNA Barcoding Teleost Fish based on the Mitochondrial Cytochrome b and Nuclear Rhodopsin Genes.|
|Authors:||BAUTISTA Jose M.; SEVILLA Rafael G.; DIEZ Amalia; NOREN Michael; MOUCHEL Olivier; JEROME Marc; VERREZ-BAGNIS Veronique; VAN PELT Hilde; FAVRE-KREY Laurence; KREY Grigorios; CARREAU Philippe; KOURTI NAOUMA; MARTINSOHN JANN|
|Citation:||MOLECULAR ECOLOGY NOTES vol. 7 p. 730-734|
|JRC Publication N°:||JRC40292|
|Type:||Articles in Journals|
|Abstract:||This report describes a set of 21 polymerase chain reaction primers and amplification conditions developed to barcode practically any teleost fish species according to their mitochondrial cytochrome b and nuclear rhodopsin gene sequences. The method was successfully tested in more than 200 marine fish species comprising the main Actinopteygii family groups. When used in phylogenetic analyses, its combination of two genes with different evolutionary rates serves to identify fish at the species level. We provide a flow diagram indicating our validated polymerase chain reaction amplification conditions for barcoding and species identification applications as well as population structure or haplotyping analyses, adaptable to high-throughput analyses.|
|JRC Institute:||Institute for the Protection and Security of the Citizen|
Files in This Item:
There are no files associated with this item.
Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.