Title: Stability of the T-DNA Flanking Regions in Transgenic Arabidopsis Thaliana Plants under Influence of Abiotic Stress and Cultivation Practices
Authors: PAPAZOVA NinaGHEDIRA RimVAN GABLEKE SabineBARTEGI AhlebWINDELS PieterTAVERNIERS IsabelROLDAN-RUIZ IsabelVAN BOCKSTAELE ErikMILCAMPS ANNEVAN DEN EEDE GUYDEPICKER AnnDE LOOSE Marc
Citation: PLANT CELL REPORTS vol. 27 no. 4 p. 749-757
Publisher: SPRINGER
Publication Year: 2008
JRC N°: JRC40524
ISSN: 0721-7714
URI: http://www.springerlink.com/content/4240l2517901mp12/
http://publications.jrc.ec.europa.eu/repository/handle/JRC40524
DOI: 10.1007/s00299-007-0495-4
Type: Articles in Journals
Abstract: Genetic transformation is often associated with different rearrangements of the plant genome at the site of insertion. Therefore the question remains weather these T-DNA insertion sites are more prone to genotoxic stresses. Here, we studied the impact of propagation through generations, the influence of gene stacking and of photo oxidative stress caused by high light intensity on the stability of the transgene and its flanking regions in the model plant Arabidopsis thaliana. Conformational Sensitive Capillary Electrophoresis (CSCE), RFLP and sequencing were deployed in this analysis in order to study the proximal 100 bp and the long range T-DNA flanking sequences. By screening seven transgenic lines no evidence for occurrence of mutation events were found, implying that the flanking regions of the studied T-DNA insertion events are relatively stable.
JRC Institute:Institute for Health and Consumer Protection

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