Title: Bioproperties of Nanocrystalline Diamond/Amorphous Carbon Composite Films
Authors: POPOV CyrilKULISCH WILHELMREITHMAIER J. P.DOSTALOVA T.JELINEK M.ANSPACH N.HAMMANN C.
Citation: DIAMOND AND RELATED MATERIALS vol. 16 no. 4-7 p. 735-739
Publisher: ELSEVIER SCIENCE SA
Publication Year: 2007
JRC N°: JRC41540
ISSN: 0925-9635
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC41540
DOI: 10.1016/j.diamond.2006.12.001
Type: Articles in Journals
Abstract: Nanocrystalline diamond/amorphous carbon (NCD/a-C) nanocomposite films have been deposited by microwave plasma chemical vapour deposition (MWCVD) from CH4/N2 mixtures. The films have been thoroughly characterized by a variety of methods with respect to their composition, morphology, structure and bonding environment. Thereafter, the bioproperties of these films have been investigated. Tests with osteoblast-like cells and pneumocytes proved that the NCD/a-C films are not cytotoxic. In addition, exposure of the films to a simulated body fluid revealed that they are bioinert. Further experiments addressed the question whether biomolecules such as RNA or proteins bind unspecifically on the surfaces of NCD/a-C films. By means of atomic force microscopy (AFM) and scanning force spectroscopy measurements it was established that, in contrast to control experiments with mica and glass, no interaction between the nanocrystalline diamond and either RNA or protein molecules took place. The results of these experiments concerning the biologically relevant properties of NCD/a-C films are discussed in view of possible future applications, e.g. as a material for the immobilization of biomolecules and their characterization by AFM measurements and related techniques.
JRC Institute:Institute for Health and Consumer Protection

Files in This Item:
There are no files associated with this item.


Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.