Title: Functionally Distinct Nucleosome-free Regions in Yeast Require Rad7 and Rad16 for Nucleotide Excision Repair
Authors: LETTIERI TERESAKRAEHENBÜHL RolfCAPIAGHI ChristophLIVINGSTONE-ZATCHEJ MagdalenaTHOMA Fritz
Citation: DNA REPAIR vol. 7 no. 5 p. 734-743
Publisher: ELSEVIER SCIENCE BV
Publication Year: 2008
JRC N°: JRC42248
ISSN: 1568-7864
URI: http://dx.doi.org/10.1016/j.dnarep.2008.01.016
http://publications.jrc.ec.europa.eu/repository/handle/JRC42248
DOI: 10.1016/j.dnarep.2008.01.016
Type: Articles in Journals
Abstract: In yeast, Rad7 and Rad16 are two proteins required for nucleotide excision repair (NER) of non-transcribed chromatin. They have roles in damage recognition, excision, as well as ultraviolet-light-dependent histone H3 acetylation. Homology of Rad16 with Snf2, the ATP-ase subunit of many chromatin remodelling complexes, suggests that Rad7-Rad16 also acts by nucleosome remodelling thereby facilitating NER. Here, we used yeast rad7¿¿rad16¿ mutants and show that Rad7-Rad16 is also required for NER of UV-lesions in three nucleosome-free regions (NFRs), the promoter and 3'-end of the URA3 gene and the ARS1 origin of replication. Moreover, rapid repair of UV-lesions by photolyase confirmed that nucleosomes were absent and that neither UV-damage formation nor rad7¿rad16¿ mutations altered chromatin structure and damage accessibility in NFRs. The data are consistent with a role of Rad7-Rad16 in damage recognition and processing in absence of nucleosomes. An additional role in nucleosome remodelling is discussed.
JRC Institute:Institute for Environment and Sustainability

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