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|Title:||Functionally Distinct Nucleosome-free Regions in Yeast Require Rad7 and Rad16 for Nucleotide Excision Repair|
|Authors:||LETTIERI TERESA; KRAEHENBÜHL Rolf; CAPIAGHI Christoph; LIVINGSTONE-ZATCHEJ Magdalena; THOMA Fritz|
|Citation:||DNA REPAIR vol. 7 no. 5 p. 734-743|
|Publisher:||ELSEVIER SCIENCE BV|
|Type:||Articles in Journals|
|Abstract:||In yeast, Rad7 and Rad16 are two proteins required for nucleotide excision repair (NER) of non-transcribed chromatin. They have roles in damage recognition, excision, as well as ultraviolet-light-dependent histone H3 acetylation. Homology of Rad16 with Snf2, the ATP-ase subunit of many chromatin remodelling complexes, suggests that Rad7-Rad16 also acts by nucleosome remodelling thereby facilitating NER. Here, we used yeast rad7¿¿rad16¿ mutants and show that Rad7-Rad16 is also required for NER of UV-lesions in three nucleosome-free regions (NFRs), the promoter and 3'-end of the URA3 gene and the ARS1 origin of replication. Moreover, rapid repair of UV-lesions by photolyase confirmed that nucleosomes were absent and that neither UV-damage formation nor rad7¿rad16¿ mutations altered chromatin structure and damage accessibility in NFRs. The data are consistent with a role of Rad7-Rad16 in damage recognition and processing in absence of nucleosomes. An additional role in nucleosome remodelling is discussed.|
|JRC Institute:||Institute for Environment and Sustainability|
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