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|Title:||Rol del Joint Research Center y el Laboratorio de Referencia Comunitario (CRL-GMFF) en la Implementacion de la Normativa Europea|
|Citation:||Actas del Seminario Internacional Sobre Organismos Geneticamente Modificados y Mercado Europeo p. 87-92|
|Publisher:||Servicio Agricola y Ganadero - SAG, Santiago (Chile)|
|JRC Publication N°:||JRC42627|
|Type:||Contributions to Conferences|
|Abstract:||The lecture will provide an overview of available approaches and methods suitable for the purpose of GMO detection, identification and quantification. The lecture will include theoretical concepts of PCR - both qualitative and quantitative real-time - and the specific requirements for their application in GMO analysis. Practical aspects will also be covered: how to search for GMOs? What to search for? Screening versus event-specific protocols, proper choice of primers and PCR format according to specific needs and legislative requirements. In presenting principles, limits and possibilities of currently available analytical tools for the purpose of GMO detection, identification and quantification, the general context will be also presented: for example: aspects to be taken into consideration before starting activities in the field of GMO analysis, requirements for proper lab implementation, importance of samples and DNA quality, minimal (or optimal) requirements for the correct conduction of the work. The lecture will also include: meaning and importance of Certified Reference Materials, proficiency-testing schemes, GLP and accreditation, etc.; the importance of method validation will also be highlighted.|
|JRC Institute:||Institute for Health and Consumer Protection|
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