Title: Stem-cell Culture on Patterned Bio-Functional Surfaces
Authors: RUIZ ANA MARIABUZANSKA LEONORACERIOTTI LAURABRETAGNOL FREDERICCOECKE SANDRACOLPO PASCALROSSI FRANCOIS
Citation: JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION vol. 19 no. 12 p. 1649-1657
Publisher: VSP BV
Publication Year: 2008
JRC Publication N°: JRC43222
ISSN: 0920-5063
URI: http://brill.publisher.ingentaconnect.com/content/vsp/bsp/2008/00000019/00000012/art00009
http://publications.jrc.ec.europa.eu/repository/handle/JRC43222
Type: Articles in Journals
Abstract: Bio-functional surfaces have been created by printing proteins on antifouling surfaces in a customised geometry. Human Umbilical Cord Neural Stem Cells incubated on the samples readily attach to the protein defined domains, where they have been monitored during 21 days of culture. The stability of the pattern varies with the density of cells anchored to the microstamped proteins. Highly packed cell patterned domains favoured non-differentiated mode, while low density areas allowed the spreading out of the cells and differentiation. Tailoring the geometry (pattern size and distances) enables improving the monitoring of the stem cells developmental processes. The biocompatible surfaces can serve as a model to study processes accompanying stem cell neural lineage commitment.
JRC Institute:Institute for Health and Consumer Protection

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