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|Title:||Development and Evaluation of Peptidic Ligands Targeting Tumor-associated Urokinase Plasminogen Activator Receptor (uPAR) for Use in Alpha-emitter Therapy of Disseminated Ovarian Cancer|
|Authors:||KNOER S.; SATO S.; HUBER T.; MORGENSTERN ALFRED; BRUCHERTSEIFER FRANK; SCHMITT M.; KESSLER H.; SENEKOWITSCH-SCHMIDTKE R.; MAGDOLEN V.; SEIDL C.|
|Citation:||EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING vol. 35 no. 1 p. 53-64|
|Type:||Articles in periodicals and books|
|Abstract:||http://www.springerlink.com/content/f4n86131k5895016/?p=86c3807e2c094db69f28dc721447fc2a&pi=8 Purpose: Among gynecologic malignancies, ovarian cancer has the highest mortality due to rapid peritoneal dissemination. Treatment failure particularly arises from failure to eliminate disseminated cells. Our aim was to develop peptidic radioligands targeting tumour cell-associated urokinase receptor (uPAR, CD87) for a-emitter therapy for advanced ovarian cancer. Methods DOTA-conjugated, uPAR-directed ligands were synthesised on solid-phase. Binding of peptides to human cells expressing uPAR was assayed by flow cytofluorometry or, in case of 213Bi-labelled peptides, by measuring cell-bound radioactivity. Bio-distribution of the 213Bi-labelled peptide P-P4D was analysed in nude mice 28 days after intraperitoneal inoculation of OV-MZ-6 ovarian cancer cells in the absence or presence of the plasma expander gelofusine. Results: uPAR-selective ligands were developed based on published high-affinity uPAR-binding peptides. For preparation of N-terminally cross-linked divalent ligands, a novel solid-phase procedure was developed. Specific binding of 213Bi-labelled peptides to monocytoid U937 and OV-MZ-6 cells was demonstrated using the natural ligand of uPAR, pro-uPA, or a soluble form of uPAR, suPAR, as competitors. The pseudo-symmetrical covalent dimer 213Bi-P-P4D displayed superior binding to OV-MZ-6 cells in vitro. Accumulation of 213Bi-P-P4D in tumour tissue was demonstrated by bio-distribution analysis in nude mice bearing intraperitoneal OV-MZ-6-derived tumours. Gelofusine reduced kidney uptake of 213Bi-P-P4D by half. Conclusion: Ovarian cancer cells overexpressing uPAR were specifically targeted in vitro and in vivo by 213Bi-P-P4D. Kidney uptake of 213Bi-P-P4D was distinctly reduced using gelofusine. Thus, this radiopeptide may represent a promising option for therapy for disseminated ovarian cancer.|
|JRC Institute:||Institute for Transuranium Elements|
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