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|Title:||Enzymatically-Tailored Pectins Differentially Influence the Morphology, Adhesion, Cell Cycle Progression and Survival of Fibroblasts|
|Authors:||NAGEL M.d.; VERHOEF R.; SCHOLS Henk; MORRA M.; KNOX J.p.; CECCONE Giacomo; DELLA VOLPE C.; VIGNERON P.; BUSSY C.; GALLET M.; VELZENBERGER E.; VAYSSADE M.; CASCARDO G.; CASSINELLI C.; HAEGER A.; GILLILAND Douglas; LIAKOS Ioannis; RODRIGUEZ-VALVERDE M.; SIBONI S.|
|Citation:||BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS vol. 1780 no. 7-8 p. 995-1003|
|Publisher:||ELSEVIER SCIENCE BV|
|Type:||Articles in periodicals and books|
|Abstract:||Improved biocompatibility and performance of biomedical devices can be achieved through the incorporation of bioactive molecules on device surfaces. Five structurally distinct pectic polysaccharides (modified hairy regions (MHRs)) were obtained by enzymatic liquefaction of apple (MHR-B, MHR-A, MHR-a), carrot (MHR-C) and potato (MHR-P) cells. Polystyrene (PS) Petri dishes, aminated by a plasma deposition process, were surface modified by the covalent linking of the MHRs. Results clearly demonstrate that MHR-B induces cell adhesion, proliferation and survival, in contrast to the other MHRs. Moreover, MHR-a causes cells to aggregate, decrease proliferation and enter into apoptosis. Cells cultured in standard conditions with 1% soluble MHR-B or MHR-a show the opposite behaviour to the one observed on MHR-B and -a-grafted PS. Fibronectin was similarly adsorbed onto MHR-B and tissue culture polystyrene (TCPS) control, but poorly on MHR-a. The Fn cell binding site (RGD sequence) was more accessible on MHR-B than on TCPS control, but poorly on MHR-a. The disintegrin echistatin inhibited fibroblast adhesion and spreading on MHR-B-grafted PS, which suggests that MHRs control fibroblast behaviour via serum adhesive proteins. This study provides a basis for the design of intelligently-tailored biomaterial coatings able to induce specific cell functions.|
|JRC Institute:||Institute for Health and Consumer Protection|
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