Title: The lamba Red Proteins Promote Efficient Recombination between Diverged Sequences: Implications for Bacteriophage Genome Mosaicism
Authors: MARTINSOHN JannRADMAN MiroslavPETIT Marie-Agnes
Citation: PLoS Genetics vol. 4 no. 5 p. e1000065
Publisher: PUBLIC LIBRARY SCIENCE
Publication Year: 2008
JRC N°: JRC46706
ISSN: 1553-7390
URI: http://www.plosgenetics.org/
http://publications.jrc.ec.europa.eu/repository/handle/JRC46706
DOI: 10.1371/journal.pgen.1000065
Type: Articles in Journals
Abstract: Genome mosaicism in temperate bacterial viruses (bacteriophages) is so great that it obscures their phylogeny at the genome level. However, the precise molecular processes underlying this mosaicism are unknown. Illegitimate recombination has been proposed, but homeologous recombination could also be at play. To test this, we have measured the efficiency of homeologous recombination between diverged oxa gene pairs inserted into ¿. High yields of recombinants between 22% diverged genes have been obtained when the virus Red Gam pathway was active, and 100 fold less when the host Escherichia coli RecABCD pathway was active. The recombination editing proteins, MutS and UvrD, showed only marginal effects on ¿ recombination. Thus, escape from host editing contributes to the high proficiency of virus recombination. Moreover, our bioinformatics study suggests that homeologous recombination between similar lambdoid viruses has created part of their mosaicism. We therefore propose that the remarkable propensity of the ¿-encoded Red and Gam proteins to recombine diverged DNA is effectively contributing to mosaicism, and more generally, that a correlation may exist between virus genome mosaicism and the presence of Red/Gam-like systems. © 2008 Martinsohn et al.
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