Title: Neutral Red Uptake Cytotoxicity Tests for Estimating Starting Doses for Acute Oral Toxicity Tests
Authors: STOKES BillCASATI SilviaSTRICKLAND JudyPARIS Michael
Citation: Current Protocols in Toxicology vol. Supplement 36 no. 20.4.1 p. 1-20
Publisher: Interscience Wiley
Publication Year: 2008
JRC Publication N°: JRC46933
ISSN: 1934-9254
URI: http://www.interscience.wiley.com
http://publications.jrc.ec.europa.eu/repository/handle/JRC46933
DOI: 10.1002/0471140856.tx2004s36
Type: Articles in Journals
Abstract: In vitro cytotoxicity assays can be used as alternative toxicity tests to reduce the total number of animals needed for acute oral toxicity tests. This unit describes two methods for determining the in vitro cytotoxicity of test substances using neutral red uptake (NRU) and using the in vitro data to determine starting doses for in vivo acute oral systemic toxicity tests, e.g., the up-and-down procedure or the acute toxic class method. The use of the NRU methods to determine starting doses for acute oral toxicity tests may reduce the number of animals required, and for relatively toxic substances, this approach may also reduce the number of animals that die or require humane euthanasia due to severe toxicity. An interlaboratory validation study has demonstrated that the methods are useful and reproducible for these purposes. Two standardized protocols provide details for performing NRU tests with rodent and human cells.
JRC Institute:Institute for Health and Consumer Protection

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