Title: Validity Assessment of the Detection Method of Maize Event Bt10 through Investigation of Its Molecular Structure
Authors: MILCAMPS AnneRABE ScottCADE RDE FRAMOND AjHENRIKSSON PeterKRAMER VPASTOR-BENITO SusanaWILLITS MgLAWRENCE DavidVAN DEN EEDE GuyLISBOA D.
Citation: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY vol. 57 no. 8 p. 3156-3163
Publisher: AMER CHEMICAL SOC
Publication Year: 2009
JRC Publication N°: JRC47065
ISSN: 0021-8561
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC47065
DOI: 10.1021/jf802627f
Type: Articles in Journals
Abstract: In March 2005, the United States authorities informed the European Commission of the inadvertent release of an unauthorized maize GM Event, Bt10, in their market and subsequently the grain channel. In the United States measures were taken to eliminate Bt10 from seed and grain supplies; in the European Union an embargo for maize gluten and brewer¿s grain import was implemented unless certified of Bt10 absence with a Bt10 specific PCR detection method. With the aim of assessing the validity of the Bt10 detection method, an in-depth analysis of the molecular organization of the genetic modification of this event was carried out by both the company Syngenta, who produced the Event, and the European Commission Joint Research Centre who validated the detection method. Using a variety of molecular analytical tools, both organizations found the genetic modification of Event Bt10 to be very complex in structure, with re-arrangements, inversions and multiple copies of the structural elements (cry1Ab, pat and the amp gene), interspersed with small genomic maize fragments. Southern blot analyses demonstrated that all Bt10 elements were found tightly linked on one large fragment, including the region that would generate the event specific PCR amplicon of the Bt10 detection method. This study proposes a hypothetical map of the insert of Event Bt10 and concludes that the validated detection method for Event Bt10 is fit for its purpose.
JRC Institute:Institute for Health and Consumer Protection

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