Title: Fabrication of Holographic Diffractive Optical Elements for Enhancing Light Collection from Fluorescence Based Biochips
Authors: MACKO PeterWHELAN Maurice
Citation: OPTICS LETTERS vol. 33 no. 22 p. 2614-2616
Publisher: OPTICAL SOC AMER
Publication Year: 2008
JRC N°: JRC47611
ISSN: 0146-9592
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC47611
DOI: 0146-9592/08/222614-3
Type: Articles in Journals
Abstract: An approach to enhancing the light collection from fluorescence emitters bound on the surface of a biochip is presented. It is based on the integration of diffractive optical elements on the underside of the chip which are essentially thick volume holograms written into a layer of photopolymer recording material. The high diffractive efficiency and angular selectivity of these type of diffractive elements make them very effective collectors of the spatially anisotropic light emitted by surface-bound fluorophores. The holographic lithography setup used to fabricate the diffractive elements is described. Results obtained using both a focused laser and light from a fluorescence spot to characterize the performance of the diffractive optical elements are presented.
JRC Institute:Institute for Health and Consumer Protection

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