Title: Micro-Stamped Surfaces for the Patterned Growth of Neural Stem Cells
Authors: RUIZ Ana MariaBUZANSKA LeonoraGILLILAND DouglasRAUSCHER HubertSIRGHI LucelSOBANSKI TomaszZYCHOWICZ MarzenaCERIOTTI LauraBRETAGNOL FredericCOECKE SandraCOLPO PascalROSSI Francois
Citation: BIOMATERIALS vol. 29 no. 36 p. 4766-4774
Publisher: ELSEVIER SCI LTD
Publication Year: 2008
JRC Publication N°: JRC47976
ISSN: 0142-9612
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC47976
DOI: 10.1016/j.biomaterials.2008.08.017
Type: Articles in Journals
Abstract: We present a method for patterning neural stem cells based on pre-patterning polypeptides on a cellrepellent surface (poly(ethylene) oxide-like, PEO-like, plasma-deposited films). The method ensures cell attachment and stability for several weeks, as well as it allows cell migration and differentiation. Various patterns of w1 nm thick cell adhesive poly-L-lysine (PLL) have been created on a cell-repellent PEO-like matrix by microcontact printing using different array configurations and printing conditions. The cell-repellent property of PEO-like film determined the confinement of the cells on the printed patterns. Optimization of the printing method showed that the most homogeneous patterns over large areas were obtained using PLL diluted in carbonate buffer (100 mM) at pH 8.4. Neural stem cells cultured on the PLL patterns in low serum and in differentiating medium over 20 days exhibited a good confinement to the polypeptide domains. The number of cells attached increased linearly with the micro-stamped PLL area. The cells were able to extend random axon-like projections to the outside of the patterns and presented high amount of ramifications when cultured in differentiating medium. Migration and axon-like outgrowth have been successfully guided by means of an interconnected squares configuration. The surfaces are suitable for controlling the patterning of stem cells and provide a platform for the assessment of the way how different cell arrangements and culture conditions influence cell interactions and cell developmental processes.
JRC Institute:Institute for Health and Consumer Protection

Files in This Item:
There are no files associated with this item.


Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.