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|Title:||Development of Real-Time PCR Assays for the Detection of Lupin Residues in Food Products|
|Authors:||GOMEZ GALAN Antonio Manuel; BROHEE Marcel; SCARAVELLI Elena; VAN HENGEL Adrianus; CHASSAIGNE Hubert|
|Citation:||EUROPEAN FOOD RESEARCH AND TECHNOLOGY vol. 230 no. 4 p. 597-608|
|Type:||Articles in Journals|
|Abstract:||Lupin is a legume that is used for human nutrition. Unfortunately lupin is known to trigger allergic reactions and therefore a mandatory declaration on the label of foodstuffs is required (Directive 2007/68/EC). To protect the allergic consumer lupin detection methods are needed. Here we present the development of two real-time polymerase chain reaction (PCR) methods that allow the detection of lupin-specific DNA. In cookies lupin could be detected at a level of only 5 mg per kg food matrix ELISA analyses were performed to compare the detection of lupin DNA with that of lupin protein, and the real-time PCR method targeting d-conglutin was shown to yield results comparable to those obtained by ELISA.|
|JRC Institute:||Institute for Reference Materials and Measurements|
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