Title: Event-specific Method for the Quantification of Cotton Line MON 1445 Using Real-timePCR
Authors: MAZZARA MarcoBOGNI AlessiaVAN DEN EEDE Guy
Publisher: OPOCE
Publication Year: 2008
JRC Publication N°: JRC48853
ISBN: 978-92-79-11052-8
ISSN: 1018-5593
Other Identifiers: EUR 23652 EN
OPOCE LB-NA-23652-EN-C
URI: http://gmo-crl.jrc.ec.europa.eu/statusofdoss.htm
http://publications.jrc.ec.europa.eu/repository/handle/JRC48853
DOI: 10.2788/4455
Type: EUR - Scientific and Technical Research Reports
Abstract: The JRC as Community Reference Laboratory for GM Food and Feed (CRL-GMFF), established by Regulation (EC) No 1829/2003, in collaboration with the European Network of GMO Laboratories (ENGL), has carried out a collaborative study to assess the performance of a quantitative event-specific method to detect and quantify the MON 1445 transformation event in cotton DNA (unique identifier MON-¿1445-2). The collaborative trial was conducted according to internationally accepted guidelines(1, 2). In accordance with Regulation (EC) No 1829/2003 of 22 September 2003 on genetically modified food and feed and with Regulation (EC) No 641/2004 of 6 April 2004 on detailed rules for the implementation of Regulation (EC) No 1829/2003, Monsanto provided the detection method and the samples (cotton seeds containing the transformation event and conventional cotton seeds). The JRC prepared the validation samples (calibration samples and blind samples at unknown GM percentage [DNA/DNA]). The collaborative trial involved twelve laboratories from nine European countries. The results of the international collaborative trial mostly met the ENGL performance requirements. The method is therefore considered applicable to the control samples provided, in accordance with the requirements of Annex I-2.C.2 to Commission Regulation (EC) No 641/2004
JRC Institute:Institute for Health and Consumer Protection

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