Title: Event-specific Method for the Quantification of Cotton Line MON 15985 Using Real-timePCR
Publisher: Publications Office of the European Union
Publication Year: 2017
JRC N°: JRC48908
ISBN: 978-92-79-70915-9
ISSN: 1831-9424
Other Identifiers: EUR 23650 EN
OP KJ-1A-23650-EN-N
URI: http://gmo-crl.jrc.ec.europa.eu/statusofdoss.htm
DOI: 10.2760/960907
Type: EUR - Scientific and Technical Research Reports
Abstract: The JRC as Community Reference Laboratory for GM Food and Feed (CRL-GMFF), established by Regulation (EC) No 1829/2003, in collaboration with the European Network of GMO Laboratories (ENGL), has carried out a collaborative study to assess the performance of a quantitative event-specific method to detect and quantify the MON 15985 transformation event in cotton DNA (unique identifier MON-15985-7). The collaborative trial was conducted according to internationally accepted guidelines (1, 2). In accordance with Regulation (EC) No 1829/2003 of 22 September 2003 on genetically modified food and feed and with Regulation (EC) No 641/2004 of 6 April 2004 on detailed rules for the implementation of Regulation (EC) No 1829/2003, Monsanto provided the detection method and the samples (cotton containing the transformation event MON 15985 and conventional cotton seeds). The JRC prepared the validation samples (calibration samples and blind samples at unknown GM percentage [DNA/DNA]). The collaborative trial involved twelve laboratories from nine European countries. The results of the international collaborative trial met the ENGL performance requirements. The method is therefore considered applicable to the control samples provided, in accordance with the requirements of Annex I-2.C.2 to Commission Regulation (EC) No 641/2004.
JRC Directorate:Institute for Health and Consumer Protection Historical Collection

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