Title: "Macromolecules to PDMS Transfer" as a General Route for PDMS Biochips
Authors: HEYRIES KevinMANDON CelineCERIOTTI LauraPONTI JessicaCOLPO PascalBLUM L.MARQUETTE Christophe
Citation: BIOSENSORS & BIOELECTRONICS vol. 24 no. 5 p. 1146-1152
Publisher: ELSEVIER ADVANCED TECHNOLOGY
Publication Year: 2009
JRC Publication N°: JRC48915
ISSN: 0956-5663
URI: http://dx.doi.org/10.1016/j.bios.2008.06.042
http://publications.jrc.ec.europa.eu/repository/handle/JRC48915
DOI: 10.1016/j.bios.2008.06.042
Type: Articles in Journals
Abstract: "Macromolecules to PDMS transfer" technique relying on the direct entrapment of macromolecules spots during PDMS polymerisation is proposed as an alternative for the easy and simple PDMS surface modification. In the present work, the development of three different applications based on this procedure is presented as proof of the method potentialities. First, C-reactive protein (CRP) sandwich immunoassay using immobilised monoclonal anti-CRP antibodies was developed for sepsis diagnosis. The preserved integrity of the immobilised monoclonal immunoglobulin permitted the sensitive detection of free CRP in human sera (LOD= 12.5µg/L, detection ranging over two decades). Then, rheumatoid arthritis diagnosis through the rheumatoid factor (RF) detection based on rabbit immunoglobulins immobilisation allowed the detection of specific antibodies in human sera samples down to low RF levels (detection range 5.3-485 IU/mL). Finally, the "Macromolecules to PDMS transfer" procedure was used to easily and rapidly produce fibronectin-based cell culture arrays. The successful attachment of HeLa and BALB/3T3 cells was demonstrated with optical microscopy and specific staining of actin and vinculin.
JRC Institute:Institute for Health and Consumer Protection

Files in This Item:
There are no files associated with this item.


Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.