Title: Event-specific Method for the Quantification of Maize Event 3272 Using Real-time PCR
Authors: CHARLES DELOBEL ChrysteleFOTI NicolettaMAZZARA MarcoVAN DEN EEDE Guy
Publisher: OPOCE
Publication Year: 2008
JRC N°: JRC48922
ISBN: 978-92-79-11045-0
ISSN: 1018-5593
Other Identifiers: EUR 23645 EN
OPOCE LB-NA-23645-EN-C
URI: http://gmo-crl.jrc.ec.europa.eu/statusofdoss.htm
http://publications.jrc.ec.europa.eu/repository/handle/JRC48922
DOI: 10.2788/43051
Type: EUR - Scientific and Technical Research Reports
Abstract: The JRC as Community Reference Laboratory for GM Food and Feed (CRL-GMFF), established by Regulation (EC) No 1829/2003, in collaboration with the European Network of GMO Laboratories (ENGL), has carried out a collaborative study to assess the performance of a quantitative event-specific method to detect and quantify the 3272 transformation event in maize DNA (unique identifier SYN-E3272-5). The collaborative trial was conducted according to internationally accepted guidelines (1, 2). In accordance with Regulation (EC) No 1829/2003 of 22 September 2003 ¿on genetically modified food and feed¿ and with Regulation (EC) No 641/2004 of 6 April 2004 ¿on detailed rules for the implementation of Regulation (EC) No 1829/2003¿, Syngenta Seeds S.A.S. provided the detection method and the samples (genomic DNA of conventional maize and of maize event 3272). The JRC prepared the validation samples (calibration samples and blind samples at unknown GM percentage [DNA/DNA]). The collaborative trial involved twelve laboratories from eleven European countries. The results of the international collaborative trial met the ENGL performance requirements. The method is therefore considered applicable to the control samples provided, in accordance with the requirements of Annex I-2.C.2 to Commission Regulation (EC) No 641/2004.
JRC Institute:Institute for Health and Consumer Protection

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