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|Title:||Nanocrystalline Diamond/Amorphous Carbon Composite Coatings for Biomedical Applications|
|Authors:||POPOV Cyril; BLIZNAKOV S.; BOYCHEVA S.; MILINOVIK N.; APOSTOLOVA M. D.; ANSPACH N.; HAMMANN C.; NELLEN W.; REITHMAIER J. P.; KULISCH Wilhelm|
|Citation:||DIAMOND AND RELATED MATERIALS vol. 17 no. 4-5 p. 882-887|
|Publisher:||ELSEVIER SCIENCE SA|
|Type:||Articles in Journals|
|Abstract:||Nanocrystalline diamond/amorphous carbon (NCD/a-C) composite films have been prepared by microwave plasma chemical vapor deposition (MWCVD) from methane/nitrogen mixtures and investigated with respect of their bioproperties. By means of atomic force microscopy (AFM) it was shown that RNA molecules can be attached to the NCD/a-C surface, which has been treated in hydrogen plasma and biochemically modified prior to the attachment. Further experiments addressed the influence of the morphology of the films on the viability and proliferation of endothelial cells. The results from scanning electron microscopy (SEM) and fluorescent microscopy revealed that homogeneous and uniform NCD/a-C films did not affect the cell shape and cytoskeleton, in contrast to films composed of individual nodules.|
|JRC Institute:||Institute for Health and Consumer Protection|
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