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|Title:||Effect of Temperature During Irradiation on the Level of Micronuclei in Human Peripheral Blood Lymphocytes Exposed to X-rays and Neutrons|
|Authors:||BRZOZOWSKA Kinga; JOHANNES Christian; OBE Gunter; HENTSCHEL Reinhard; MORAND Josselin; MOSS Raymond; WITTIG Andrea; SAUERWEIN Wolfgang; LINIECKI Julien; SZUMIEL Irena; WOJCIK Andrzej|
|Citation:||INTERNATIONAL JOURNAL OF RADIATION BIOLOGY vol. 85 no. 10 p. 891-899|
|Publisher:||TAYLOR & FRANCIS LTD|
|Type:||Articles in Journals|
|Abstract:||Objectives: It has been reported that the level of cytogenetic damage in human peripheral blood lymphocytes (PBL) is higher following irradiation at 378C than at 0-48C. The mechanisms of this cytogenetic temperature effect are not fully known. The aim of our study was to check whether the effect was related to the indirect or direct action of radiation. Materials and methods: PBL were kept at 378C and 08C for 20 min and exposed to 2 Gy of X-rays. In some experiments PBL were isolated and 0.5 Mdimethyl sulfoxide (DMSO) was added for 5 min before exposure. PBL were also irradiated at 378C and 08C with 1 Gy of 6 MeV neutrons. Micronuclei were scored as the endpoint. Following exposure to X-rays the level of initial DNA damage was also measured by the alkaline and neutral comet assay. Results: The frequency of micronuclei in cells exposed at 378C to X-rays or neutrons was higher than that after exposure at 08C. No effect of temperature was seen when PBL were exposed to X-rays in the presence of DMSO. No effect of temperature was observed on the level of DNA damage measured with the alkaline or neutral comet assay. Conclusions: The results of experiments with DMSO indicate that the temperature effect is due to the indirect action of radiation, i.e., via reactive oxygen species. However, this is not supported by the results with neutrons and the comet assay. Possible reasons for the discrepancies are discussed.|
|JRC Institute:||Institute for Energy and Transport|
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