Title: Water Quality Assessment by Multiparameters Flow Citometry
Authors: PREMAZZI GuidoZANON G.
Citation: Cytometry, J. of Soc. for Anal. Cytology no. Supplement 1
Publication Year: 1987
JRC N°: JRC5028
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC5028
Type: Contributions to Conferences
Abstract: WATER QUALITY CONTROL UTILISES ALGAL BIOASSAYS TO STUDY THE EFFECTS OF CHEMICAL POLLUTANTS ON WATER BODIES. SUCH RESEARCH NEEDS TO MAKE LABORATORY SYSTEMS CLOSER RESEMBLE NATURAL ECOSYSTEMS, THE FIRST STEP BEING TO STUDY MIXED ALGAL POPULATIONS. A HOME-BUILT MULTIPARAMETER INSTRUMENT HAS BEEN USED IN THE FIRST SUCH FLOW CYTOMETRIC STUDIES ON FRESHWATER ALGAL POPULATIONS, MEASURING MULTI-ANGLE LIGHT SCATTER FOR MORPHOLOGICAL DISTINCTION AND TWO WAVELENGHT FLUORESCENCE EMISSION FOR TOXONOMIC DISCRIMINATION. A TOXICOLOGI- CAL STUDY WAS MADE ON THE EFFECTS OF ENVIRONMENTAL CONCENTRATIONS OF TRACE METALS (E.G. 1-10 MG/1 CU OR CD) ON FIVE ALGAE (PANDORINA MORUM, PEDIASTRUM BORYANUM, SELENASTRUM CAPRICORNUTUM, STAUVERSTRUM CINGULUM, STICHOCOCCUS SP.) QUANTIFIED BY THE ES50 AFTER 96 H. SIGNIFICANT GREATER EFFECTS WERE OBTAINED USING CYTOMETRIC DATA WHICH ACCOUNTS FOR THE DEAD POPULATION FRACTION VIA CHLOROPHYLL AUTOFLUORESCENCE (E.G. EC50 OF CU FOR PEDIASTRUM. COULTER - 190 MG/1, CYTOMETER - 100 MG/1, EC50 OF CD FOR STAURASTRUM. COULTER - 18.5 MG/1, CYTOMETER - 1.8 MG/1). A SECOND STUDY SHOWED THAT PREDICTED ENVIRONMENTAL CONCENTRATIONS (10-100 MG/1) OF NITRILOTRIACETIC ACID (NTA) CAUSE A SHIFT TO DOMINANCE OF TWO GREEN ALGAE (PANDORINA, SELENASTRUM) OVER A BLUE-GREEN (OSCIL- LATORIA REDEKEI) IN SYNTHETIC MEDIUM (FROM 52% BIOVOLUME GREENS, 48% BLUE-GREEN IN THE CONTROL TO 72% GREENS, 28% BLUE-GREEN IN 100 MG/1 NTA). NO CHANGE IN RELATIVE CELL NUMBERS WAS OBSERVED AND NO SUCH SHIFT WAS EVIDENT WHEN THE EXPERIMENT WAS REPEATED IN A NATURAL MEDIUM. THE ADVANTAGE OF MULTI-SPECIES BIOASSAYS IS NOTEABLE SINCE NO SINGLE SPECIES APPEARED UNIVERSALLY MOST- SENSITIVE TO THE CONTAMINANTS STUDIED
JRC Institute:Joint Research Centre Historical Collection

Files in This Item:
There are no files associated with this item.


Items in repository are protected by copyright, with all rights reserved, unless otherwise indicated.