Title: A PCR-microarray method for the screening of genetically modified organisms
Authors: HAMELS SandrineGLOUDEN ThomasGILLARD KarineMAZZARA MarcoDEBODE FrédéricFOTI NicolettaSNEYERS MyriamESTEVE NUEZ TeresaPLA MariaBERBEN GilbertMOENS WilliamBERTHEAU YvesAUDEON ColetteVAN DEN EEDE GuyREMACLE Josè
Citation: EUROPEAN FOOD RESEARCH AND TECHNOLOGY vol. 228 no. 4 p. 531-541
Publisher: SPRINGER
Publication Year: 2009
JRC Publication N°: JRC50383
ISSN: 1438-2377
URI: http://springerlink.com/content/lj848673195767uu/?p=668cac55428d4b86ac69bbe33eb7613a&pi=3
http://publications.jrc.ec.europa.eu/repository/handle/JRC50383
DOI: 10.1007/s00217-008-0960-5
Type: Articles in Journals
Abstract: A new method to screen and to identify genetically modified organisms (GMO) is presented in this paper. It is based on the detection of multiple genetic elements common to GMO by their amplification via PCR followed by direct hybridisation of the amplicons on microarray. The pattern of the elements is then compared to a database of the composition of EU-approved GMO and an identification of the GMO is then proposed. The limit of detection of the method was B0.1% GMO content (w/w) expressed as the amount of target DNA present in the template for single unprocessed material. The DNA targets were detected both in reference materials and in mixtures with the same detection limit. The specificity for the detection of the different elements was found to be very good with no crossreaction even in samples with two GMO present at different concentrations. The paper presents examples of GMO identification and discusses the potential and limitation of such approaches and how they can facilitate the work of private and enforcement detection laboratories.
JRC Institute:Institute for Health and Consumer Protection

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