Title: Optimisation and Validation of Programmed Temperature Vaporization (PTV) Injection in Solvent Vent Mode for the Analysis of the 15 + 1 EU-Priority PAHs by GC¿MS
Authors: GOMEZ RUIZ Jose AngelCORDEIRO RAPOSO FernandoLOPEZ SANCHEZ PatriciaWENZL Thomas
Citation: TALANTA vol. 80 no. 2 p. 643-650
Publisher: ELSEVIER SCIENCE BV
Publication Year: 2009
JRC Publication N°: JRC52353
ISSN: 0039-9140
URI: http://www.elsevier.com/locate/talanta
http://publications.jrc.ec.europa.eu/repository/handle/JRC52353
DOI: 10.1016/j.talanta.2009.07.041
Type: Articles in Journals
Abstract: This paper presents the optimisation of a programmed temperature vaporization solvent vent (PTV-SV) injection gas chromatographic mass spectrometric (GC-MS) method for the analysis of the 15 + 1 EU-priority PAHs in food extracts. Three operation parameters (vent time, vent flow and vent pressure) were optimised by applying a D-optimal experimental design. Among these variables, vent time showed the highest effect on the analytical response (signal intensity) of the target PAHs. The 15 + 1 EU-priority PAHs were analysed in solvent solutions and in extracts of fortified sausage. In addition, blank lamb meat extracts were prepared and spiked with the target PAHs prior to GC-MS analysis. The performance of the optimised PTV-SV injection GC-MS method was scrutinised for linearity, precision, matrix effects and robustness. All parameters were found satisfactorily. Compared to PTV injection in splitless mode, the PTV-SV injection method provided an enhancement of sensitivity for all target PAHs. Especially significant was the improvement of the S/N ratios of the compounds with the highest molecular mass.
JRC Institute:Institute for Reference Materials and Measurements

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