Title: Light Collection from Fluorescence-Based Biochips by Holographic Diffractive Optical Elements
Authors: MACKO PeterWHELAN Maurice
Citation: Proceedings of SPIE vol. 7371 p. 73711E
Publisher: SPIE
Publication Year: 2009
JRC Publication N°: JRC52521
URI: http://dx.doi.org/10.1117/12.831730
http://publications.jrc.ec.europa.eu/repository/handle/JRC52521
DOI: 10.1117/12.831730
Type: Contributions to Conferences
Abstract: A fluorescence-based biochip with an integrated holographic diffractive optical element on its underside is presented. The diffractive element is a thick volume hologram written into a layer of photopolymer recording material. The element acts as a collector of the spatially anisotropic fluorescence light emitted from surface-bound fluorophores and redirects the light to a CCD detector. The holographic lithography setup used to fabricate the diffractive elements is described. The performance of the diffractive elements to enhance the light collection is demonstrated.
JRC Institute:Institute for Health and Consumer Protection

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