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|Title:||Transcriptomic-based Bioassays for the Detection of Type A Trichothecenes|
|Authors:||LANCOVA Katarina; BOWENS Priscilla; STROKA Joerg; GMUENDER Hans; ELLINGER T.; NAEGELI Hans-Peter|
|Citation:||World Mycotoxin Journal vol. 2 no. 2 p. 247-257|
|Publisher:||Wageningen Academic Publishers|
|Type:||Articles in Journals|
|Abstract:||The type A trichothecenes T-1 toxin (T-2) and HT-2 toxin (HT-2) are hazardous Fusarium products that contaminate many field crops growing in cold to temperate regions across the world. Toxicity studies in laboratory and farm animals have been used to derive a temporary tolerable daily intake (t-TDI) for the sum of T-2 and HT-2 of no more than 60 ng/kg body weight. To protect the consumers, it is now necessary to screen a large number of food samples for the presence of these poisonous fungal metabolites. Towards that goal, we discovered that the transcriptional apparatus of a human carcinoma cell line (MCF7) provides a sensitive biological sensor of type A trichothecenes. In fact, exposure of this easy-to-culture cell line to T-2 and HT-2 results in the regulation of >2,000 different transcripts with expressin changes ranging from >5,000-fold gene inductions to >40-fold gene repressions. These transcriptional responses have been exploited to develop practival microship and reverse transcription-polymerase chain reaction (RT-PCR) assays for the detection of type A trichothecenes at parts per billion levels.|
|JRC Institute:||Institute for Reference Materials and Measurements|
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