Title: A Quantitative In Vitro Approach to Study the Intracellular Fate of Gold Nanoparticles: From Synthesis to Cytotoxicity
Authors: PONTI JessicaCOLOGNATO RenatoFRANCHINI FabioGIORIA SabrinaSIMONELLI FedericaABBAS KamelUBOLDI C.KIRKPATRICK C. J.HOLZWARTH UweROSSI Francois
Citation: NANOTOXICOLOGY vol. 3 no. 4 p. 296-306
Publisher: INFORMA HEALTHCARE
Publication Year: 2009
JRC Publication N°: JRC54462
ISSN: 1743-5390
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC54462
DOI: 10.1080/17435390903056384
Type: Articles in Journals
Abstract: Due to their physico-chemical characteristics, gold nanoparticles (AuNPs) seem to be suitable for biomedical and therapeutic applications even if conflicting data on their toxicological profiles are present in literature. In order to better understand if AuNPs could be safe we must consider different biological endpoints such as cytotoxicity, genotoxicity, inflammation and biopersistence. Starting from these considerations, one of the first issues to be assessed is to better understand if AuNPs can be internalized by cells. In this work, we propose a methodological approach to radioactivate AuNPs by neutron activation and the quantification of their internalization by two in vitro cell systems such as MDCK and HepG2 after 24 h of exposure. Despite a dose-dependent internalization, no evidence of cytotoxicity, determined by two different standard in vitro methods such as Neutral Red Uptake and Colony Forming Efficiency, was observed.
JRC Institute:Institute for Health and Consumer Protection

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