Title: Validation Procedures for Quantitative Food Allergen ELISA Methods: Community Guidance and Best Practices
Authors: ABBOTT MichaelHAYWARD StephenROSS WilliamGODEFROY Samuel BenrejebULBERTH FranzVAN HENGEL ArjonROBERTS JamesAKIYAMA HiroshiPOPPING BertYEUNG Jupiter M.WEHLING PaulTAYLOR S.l.POMS Roland ernestDELAHAUT Philippe
Citation: JOURNAL OF AOAC INTERNATIONAL vol. 93 no. 2 p. 442-450
Publisher: AOAC INT
Publication Year: 2010
JRC N°: JRC54979
ISSN: 1060-3271
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC54979
Type: Articles in Journals
Abstract: This document provides supplement guidance on specifications for the development and implementation of studies to validate the performance characteristics of quantitative ELISA methods for the determination of food allergens. It is intended as a companion document to the "AOAC Official Methods of Analysis Appendix D: Guidelines for Collaborative Study Procedures to Validate Characteristics of a Method of Analysis" www.aoac.org (1). The guidance is divided into two sections: information to be provided by the method developer on various characteristics of the method, and implementation of a multi-laboratory validation study. Certain criteria included in the guidance are allergen-specific. Two food allergens: egg and milk are used to demonstrate the criteria guidance. These recommendations will be the basis of the harmonized validation protocol for any food allergen ELISA method, whether proprietary or nonproprietary, that will be submitted to AOAC and/or regulatory authorities or other bodies for status recognition. It should be noted that regulatory authorities may have their own particular requiretments for data packages in addition to the guidance in this document. Future work is planned for the implementation and validation of this guidance. This future work will also inclkude guidance specific to other priority allergens
JRC Institute:Institute for Reference Materials and Measurements

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