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|Title:||Evidence of 13C non-covalent isotope effects obtained by quantitative 13C nuclear magnetic resonance spectroscopy at natural abundance during normal phase liquid chromatography|
|Authors:||BOTOSOA Eliot P.; SILVESTRE Virginie; ROBINS Richard; MORENO-ROJAS Jose Manuel; GUILLOU Claude; REMAUD Gerald|
|Citation:||JOURNAL OF CHROMATOGRAPHY A vol. 1216 no. 42 p. 7043-7048|
|Publisher:||ELSEVIER SCIENCE BV|
|Type:||Articles in Journals|
|Abstract:||Quantitative isotopic 13 C NMR at natural abundance has been used to determine the site-by-site 13 C/12 C ratios in vanillin and a number of related compounds eluted from silica gel chromatography columns under similar conditions. Head-to-tail isotope fractionation is observed in all compounds at the major- ity of carbon positions. Furthermore, the site-specific isotope deviations show signatures characteristic of the position and functionality of the substituents present. The observed effects are more complex than would be obtained by simply summing the individual effects. Such detail is hidden when only the global 13 C content is measured by mass spectrometry. In particular, carbon positions within the aromatic ring are found to show site-specific isotope fractionation between the solute and the stationary phase. These interactions, defined as non-covalent isotope effects, can be normal or inverse and vary with the substitution pattern present.|
|JRC Institute:||Institute for Health and Consumer Protection|
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