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|Title:||X-Ray Spectromicroscopy Study of Ubiquitin Adsorption to Plasma Polymerized Microstructures|
|Authors:||CECCONE Giacomo; LEUNG B.; PEREZ ROLDAN MARIA; VALSESIA Andrea; COLPO Pascal; ROSSI Francois; HITCHCOCK A.; SCHOLL A.|
|Citation:||SURFACE AND INTERFACE ANALYSIS vol. 42 no. 6-7 p. 830-834|
|Publisher:||JOHN WILEY & SONS LTD|
|Type:||Articles in Journals|
|Abstract:||Synchrotron-based X-ray PhotoEmission Electron Microscopy (X-PEEM), Atomic Force Microscopy (AFM), time of flight secondary ion mass spectrometry (ToF SIMS) and X-ray photoelectron spectroscopy (XPS) have been used to visualize and characterize plasma polymerized acrylic acid (ppAA) and poly ethylene oxide (PEO)-like microstructures. Micropatterned microstructures have been fabricated with electron-beam lithography to obtain contrasted ppAA/PEO-like structures. A surface area of 500 µm× 500 µm was patterned with arrays of 5 µm diameter circles and 500 µm x 5 µm lines. Samples have been analyzed before and after immersion of ubiquitin protein solutions. AFM and X-PEEM results indicated that proteins adsorbed preferentially on the ppAA regions. For both circular and line patterns, the adsorbed protein preferentially adsorbed to the ppAA regions.|
|JRC Institute:||Institute for Health and Consumer Protection|
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