Title: X-Ray Spectromicroscopy Study of Ubiquitin Adsorption to Plasma Polymerized Microstructures
Authors: CECCONE GiacomoLEUNG B.PEREZ ROLDAN MARIAVALSESIA AndreaCOLPO PascalROSSI FrancoisHITCHCOCK A.SCHOLL A.
Citation: SURFACE AND INTERFACE ANALYSIS vol. 42 no. 6-7 p. 830-834
Publisher: JOHN WILEY & SONS LTD
Publication Year: 2010
JRC N°: JRC56704
ISSN: 0142-2421
URI: www.interscience.wiley.com
http://publications.jrc.ec.europa.eu/repository/handle/JRC56704
DOI: 10.1002/sia.3239
Type: Articles in Journals
Abstract: Synchrotron-based X-ray PhotoEmission Electron Microscopy (X-PEEM), Atomic Force Microscopy (AFM), time of flight secondary ion mass spectrometry (ToF SIMS) and X-ray photoelectron spectroscopy (XPS) have been used to visualize and characterize plasma polymerized acrylic acid (ppAA) and poly ethylene oxide (PEO)-like microstructures. Micropatterned microstructures have been fabricated with electron-beam lithography to obtain contrasted ppAA/PEO-like structures. A surface area of 500 µm× 500 µm was patterned with arrays of 5 µm diameter circles and 500 µm x 5 µm lines. Samples have been analyzed before and after immersion of ubiquitin protein solutions. AFM and X-PEEM results indicated that proteins adsorbed preferentially on the ppAA regions. For both circular and line patterns, the adsorbed protein preferentially adsorbed to the ppAA regions.
JRC Institute:Institute for Health and Consumer Protection

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