Title: The Third SeaWiFS HPLC Analysis Round-Robin Experiment (SeaHARRE-3)
Authors: HOOKER StanfordVAN HEUKELEM LaurieTHOMAS CrystalCLAUSTRE HervéRAS JoséphineSCHLUTER LouiseCLEMENTSON LesleyVAN DER LINDE DirkEKER-DEVELI ElifBERTHON Jean-FrancoisBARLOW RaySESSIONS HeatherISMAIL HassanPERL Jason
Publisher: Goddard Space Flight Center
Publication Year: 2009
JRC N°: JRC57305
URI: http://publications.jrc.ec.europa.eu/repository/handle/JRC57305
Type: Articles in books
Abstract: Seven international laboratories specializing in the determination of marine pigment concentrations using high performance liquid chromatography (HPLC) were intercompared using in situ samples and a mixed pigment sample. The field samples were collected primarily from oligotrophic waters, although mesotrophic and eutrophic waters were also sampled to create a dynamic range in chlorophyll concentration spanning approximately two orders of magnitude (0.020-1.366 mg m^-3). The intercomparisons were used to establish the following: a) the uncertainties in quantitating individual pigments and higher-order variables (sums, ratios, and indices); b) the reduction in uncertainties as a result of applying quality assurance (QA) procedures; c) the importance of establishing a properly defined referencing system in the computation of uncertainties; d) the analytical benefits of performance metrics, and e) the utility of a laboratory mix in understanding method performance. In addition, the remote sensing requirements for the in situ determination of total chlorophyll a were investigated to determine whether or not the average uncertainty for this measurement is being satisfied.
JRC Institute:Institute for Environment and Sustainability

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