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|Title:||The Third SeaWiFS HPLC Analysis Round-Robin Experiment (SeaHARRE-3)|
|Authors:||HOOKER Stanford; VAN HEUKELEM Laurie; THOMAS Crystal; CLAUSTRE Hervé; RAS Joséphine; SCHLUTER Louise; CLEMENTSON Lesley; VAN DER LINDE Dirk; EKER-DEVELI Elif; BERTHON Jean-Francois; BARLOW Ray; SESSIONS Heather; ISMAIL Hassan; PERL Jason|
|Publisher:||Goddard Space Flight Center|
|Type:||Articles in periodicals and books|
|Abstract:||Seven international laboratories specializing in the determination of marine pigment concentrations using high performance liquid chromatography (HPLC) were intercompared using in situ samples and a mixed pigment sample. The field samples were collected primarily from oligotrophic waters, although mesotrophic and eutrophic waters were also sampled to create a dynamic range in chlorophyll concentration spanning approximately two orders of magnitude (0.020-1.366 mg m^-3). The intercomparisons were used to establish the following: a) the uncertainties in quantitating individual pigments and higher-order variables (sums, ratios, and indices); b) the reduction in uncertainties as a result of applying quality assurance (QA) procedures; c) the importance of establishing a properly defined referencing system in the computation of uncertainties; d) the analytical benefits of performance metrics, and e) the utility of a laboratory mix in understanding method performance. In addition, the remote sensing requirements for the in situ determination of total chlorophyll a were investigated to determine whether or not the average uncertainty for this measurement is being satisfied.|
|JRC Institute:||Sustainable Resources|
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